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Data preparation and processing The raw sequencing files were demultiplexed, quality filtered, denoised, purged from chimeras and clustered into OTUs based on the 97% similarity criterion, using the OptiClust algorithm 38 with the software MOTHUR (v.1.48.0 39 ) and classified with the SILVA database (v138.1 40 )
The only sticking point is that the top hook is a little harder to use and I'd be worried about my dexterity on a cold mountaintop
Simply begin reeling in your line, and the fish will hook itself in the corner of its mouth
Usual wear and tear but in great condition
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